streptococcus pneumoniae strains d-39 and atcc8  (BEI Resources)

Journal: Investigative Ophthalmology & Visual Science

Article Title: Antibodies to Conserved Surface Polysaccharides Protect Mice Against Bacterial Conjunctivitis

doi: 10.1167/iovs.18-23795

Figure Lengend Snippet: Intraperitoneal administration of MAb to PNAG preinfection and postinfection reduces bacterial burdens and conjunctival pathology due to S. pneumoniae (D-39 and ATCC8) and S. aureus (LAC) after 48 hours of infection in A/J mice. Eighteen hours prior to infection and 4, 24, and 32 hours postinfection, 200 μg of the human IgG1 monoclonal antibody MAb F598 to PNAG or control IgG1 MAb F429 were injected IP. Mice (n = 8 per group) were euthanized at 48 hours postinfection and pathology scores (upper row) and CFU/conjunctiva (lower row) were determined. Symbols represent individual animals, black lines the median, and gray lines the 95% CI, and one-sided P values were determined by nonparametric t-tests.

Article Snippet: Streptococcus pneumoniae strains D-39 and ATCC8 were obtained from BEI Resources (Manassas, VA, USA) and grown in Todd-Hewitt Broth (BD Difco, Houston, TX, USA) with 1% added glucose statically in a 5% CO 2 incubator until they reached the mid-log phase of growth.

Techniques: Infection, Control, Injection

Journal: Investigative Ophthalmology & Visual Science

Article Title: Antibodies to Conserved Surface Polysaccharides Protect Mice Against Bacterial Conjunctivitis

doi: 10.1167/iovs.18-23795

Figure Lengend Snippet: Postinfection intraconjunctival administration of MAb to PNAG reduces bacterial burdens and conjunctival pathology due to S. pneumoniae (strains D-39 and ATCC8) and S. aureus (strain LAC) after 48 hours of conjunctival infection in A/J mice. A total of 10 μg of control IgG MAb (□) or MAb to PNAG (▵) were injected into the conjunctiva 4, 24, and 32 hours postinfection. Mice (n = 8 per group) were euthanized at 48 hours postinfection and pathology scores (upper row) and CFU/conjunctiva (lower row) were determined. Symbols represent individual animals, black lines the median, and gray lines the 95% CI, and one-sided P values were determined by nonparametric t-tests.

Article Snippet: Streptococcus pneumoniae strains D-39 and ATCC8 were obtained from BEI Resources (Manassas, VA, USA) and grown in Todd-Hewitt Broth (BD Difco, Houston, TX, USA) with 1% added glucose statically in a 5% CO 2 incubator until they reached the mid-log phase of growth.

Techniques: Infection, Control, Injection

Journal: Investigative Ophthalmology & Visual Science

Article Title: Antibodies to Conserved Surface Polysaccharides Protect Mice Against Bacterial Conjunctivitis

doi: 10.1167/iovs.18-23795

Figure Lengend Snippet: Postinfection topical administration of MAb to PNAG reduces bacterial burdens and conjunctival pathology due to S. pneumoniae (D-39 and ATCC8) and S. aureus (LAC) after 48 hours of infection in A/J mice. A total of 10 μg of control IgG MAb (□) or MAb to PNAG (▵) were administered topically onto conjunctiva 4, 24, and 32 hours postinfection. Mice (n = 7 to 8 per group) were euthanized at 48 hours post infection and pathology scores (upper row) and CFU/conjunctiva (lower row) determined. Symbols represent individual animals, black lines the median, and gray lines the 95% CI, and one-sided P values were determined by nonparametric t-tests.

Article Snippet: Streptococcus pneumoniae strains D-39 and ATCC8 were obtained from BEI Resources (Manassas, VA, USA) and grown in Todd-Hewitt Broth (BD Difco, Houston, TX, USA) with 1% added glucose statically in a 5% CO 2 incubator until they reached the mid-log phase of growth.

Techniques: Infection, Control

Journal: Investigative Ophthalmology & Visual Science

Article Title: Antibodies to Conserved Surface Polysaccharides Protect Mice Against Bacterial Conjunctivitis

doi: 10.1167/iovs.18-23795

Figure Lengend Snippet: Postinfection administration of MAb to PNAG reduces bacterial burdens and conjunctival pathology due to S. pneumoniae (D-39 and ATCC8) or S. aureus (LAC) in WT C57Bl/6, but not in RAG 1 KO or germ-free mice (GFM) after 48 hours. A total of 10 μg of control IgG MAb (□) or MAb to PNAG (▵) were injected into the conjunctiva 4, 24, and 32 hours postinfection. Mice (n = 5 per group) were euthanized at 48 hours postinfection and pathology scores (upper row) and CFU/conjunctiva (lower row) were determined. Symbols represent individual animals, bars the median, and error bars the 95% CI, and one-sided P values were determined by nonparametric t-tests. None of the differences between MAb to PNAG or control MAb in RAG 1 KO or GFM mice were significant (P > 0.22).

Article Snippet: Streptococcus pneumoniae strains D-39 and ATCC8 were obtained from BEI Resources (Manassas, VA, USA) and grown in Todd-Hewitt Broth (BD Difco, Houston, TX, USA) with 1% added glucose statically in a 5% CO 2 incubator until they reached the mid-log phase of growth.

Techniques: Control, Injection

Journal: Investigative Ophthalmology & Visual Science

Article Title: Antibodies to Conserved Surface Polysaccharides Protect Mice Against Bacterial Conjunctivitis

doi: 10.1167/iovs.18-23795

Figure Lengend Snippet: Postinfection administration of MAb to PNAG reduces conjunctival PMN infiltration due to S. pneumoniae (D-39 and ATCC8) and S. aureus (LAC) after 48 hours of conjunctival infection in A/J mice. A total of 10 μg of control IgG MAb or MAb to PNAG were injected into the conjunctiva 4, 24, and 32 hours postinfection in mice infected with S. pneumoniae D-39 or ATCC8 or S. aureus LAC. Control MAb-treated mice showed a large infiltrate of inflammatory cells and obvious edema in conjunctival areas, whereas mice treated with MAb to PNAG had only low levels of inflammatory cells and little edema. Arrows point to site of injection in the conjunctiva. Green bars: 10 μm. Boxed areas, when shown, indicated magnified area.

Article Snippet: Streptococcus pneumoniae strains D-39 and ATCC8 were obtained from BEI Resources (Manassas, VA, USA) and grown in Todd-Hewitt Broth (BD Difco, Houston, TX, USA) with 1% added glucose statically in a 5% CO 2 incubator until they reached the mid-log phase of growth.

Techniques: Infection, Control, Injection

Journal: Investigative Ophthalmology & Visual Science

Article Title: Antibodies to Conserved Surface Polysaccharides Protect Mice Against Bacterial Conjunctivitis

doi: 10.1167/iovs.18-23795

Figure Lengend Snippet: Postinfection administration of MAb to PNAG reduces conjunctival myeloperoxidase due to S. pneumoniae (D-39 and ATCC8) and S. aureus (LAC) after 48 hours of conjunctival infection in A/J mice. A total of 10 μg of control IgG MAb (□) or MAb to PNAG (▵) were injected into the conjunctiva 4, 24, and 32 hours postinfection with S. pneumoniae (D-39, ATCC8) or S. aureus LAC. Mice were euthanized at 48 hours postinfection and MPO/conjunctiva was determined. Symbols represent individual animals, black lines the medians, and gray lines the 95% CI, and P values were determined by one-sided nonparametric t-tests.

Article Snippet: Streptococcus pneumoniae strains D-39 and ATCC8 were obtained from BEI Resources (Manassas, VA, USA) and grown in Todd-Hewitt Broth (BD Difco, Houston, TX, USA) with 1% added glucose statically in a 5% CO 2 incubator until they reached the mid-log phase of growth.

Techniques: Infection, Control, Injection